Composite

Part:BBa_K238004:Design

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-07-15)


Vanillin synthesis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 895
    Illegal BamHI site found at 1297
    Illegal BamHI site found at 6341
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 638
    Illegal NgoMIV site found at 1682
    Illegal NgoMIV site found at 1690
    Illegal NgoMIV site found at 2440
    Illegal NgoMIV site found at 2613
    Illegal NgoMIV site found at 3015
    Illegal NgoMIV site found at 3019
    Illegal AgeI site found at 585
    Illegal AgeI site found at 754
    Illegal AgeI site found at 4929
    Illegal AgeI site found at 5466
    Illegal AgeI site found at 5927
    Illegal AgeI site found at 6369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 189
    Illegal BsaI site found at 455
    Illegal BsaI.rc site found at 2214
    Illegal BsaI.rc site found at 3846


Design Notes

parts for fcs (BBa_I742114) and ech (BBa_I742112) will probably not be used and made from another sequence.


Source

most parts were found in the registry.

References